No bind-wash-elute, enzymes, or use of magnetic beads!
The Diffinity RapidTip functional pipette tip contains everything you need for PCR purification. The tip is filled with our proprietary adsorption technology that has a differential affinity for PCR reaction components. The impurities (e.g., single-stranded primers and nucleotides used in the PCR process) are removed from the solution as it enters the pipette tip. Dispensing the solution yields purified, high quality DNA ready to use in your next application. You don’t need to use any capital equipment, reagents or buffers, or enzymes — all you need is a pipettor!
The functional pipette tip will fit on most conventional single or multiple, manual or automated laboratory pipettors. Samples containing DNA and impurities are aspirated into the tip where the impurities are adsorbed onto proprietary surfaces within the tip by mixing for approximately one minute. Expelling the solution yields purified DNA, the desired product.